--- title: SeqGen Blog | LC-MS description: LC-MS | --- - [MARKETPLACE](https://seqgen.com/marketplace/) - [CAREERS](https://seqgen.com/careers/) - [TERMS & CONDITIONS](https://seqgen.com/wp-content/uploads/2026/03/POL_011-Rev.A_Terms-and-Conditions-of-Sales-for-Products-and-Services.pdf) - [ISO 9001 CERTIFICATE](https://seqgen.com/wp-content/uploads/2026/01/SeqGen-Final-Cert_2026-2029.pdf) - [SALES INQUIRY](https://seqgen.com/sales-inquiry/) - [SERVICE REQUEST](http://seqgen.com/service-request/) - [TESTIMONIALS](https://seqgen.com/testimonials/) [![SeqGen](https://seqgen.com/wp-content/uploads/2020/03/seqgen_logo.png)](https://seqgen.com/) - [SeqMo Rewards](https://seqgen.com/seqmo-rewards/) - [HOME](https://seqgen.com/) - [ABOUT US](https://seqgen.com/about-us/) - [Our History](https://seqgen.com/about-us/our-history-2/) - [Our Engineers](https://seqgen.com/about-us/our-engineers/) - INSTRUMENT MODEL - [LC/MS](https://seqgen.com/instrument-model/lc-ms/) - [Sciex 3000 system](https://seqgen.com/instrument-model/lc-ms/api-3000/) - [Sciex 3200 system](https://seqgen.com/instrument-model/lc-ms/api-3200/) - [Sciex 4000 system](https://seqgen.com/instrument-model/lc-ms/api-4000/) - [Sciex 4500 system](https://seqgen.com/instrument-model/lc-ms/api-4500/) - [Sciex 5500 system](https://seqgen.com/instrument-model/lc-ms/api-5500/) - [Sciex 6500 system](https://seqgen.com/instrument-model/lc-ms/api-6500/) - [LC systems](https://seqgen.com/instrument-model/lc-ms/lc-systems/) - [DNA Analyzer](https://seqgen.com/instrument-model/dna-analyzer/) - [ABI 3100](https://seqgen.com/instrument-model/dna-analyzer/abi-3100/) - [ABI 3130/3130XL](https://seqgen.com/instrument-model/dna-analyzer/abi-3130-3130xl/) - [ABI 3500/3500XL](https://seqgen.com/instrument-model/dna-analyzer/abi-3500-3500xl/) - [ABI 3730/3730XL](https://seqgen.com/instrument-model/dna-analyzer/abi-3730-3500xl/) - [Real-Time PCR](https://seqgen.com/instrument-model/real-time-pcr/) - [ABI 7300/7500](https://seqgen.com/instrument-model/real-time-pcr/abi-7300-7500/) - [ABI 7900](https://seqgen.com/instrument-model/real-time-pcr/abi-7900/) - [Thermal Cyclers](https://seqgen.com/instrument-model/thermal-cyclers/) - [ABI 2720](https://seqgen.com/instrument-model/thermal-cyclers/abi-2720-2/) - [ABI 9700](https://seqgen.com/instrument-model/thermal-cyclers/abi-9700/) - [ABI 9800](https://seqgen.com/instrument-model/thermal-cyclers/abi-9800/) - [Liquid Handler](https://seqgen.com/instrument-model/liquid-handler/) - [Tecan Freedom Evo](https://seqgen.com/instrument-model/liquid-handler/tecan-freedom-evo/) - [Microplate Reader](https://seqgen.com/instrument-model/microplate-reader/) - [Coming Soon](https://seqgen.com/instrument-models/microplate-reader/) - [SALES](https://seqgen.com/sales/) - [SERVICE](https://seqgen.com/service/) - [CONTACT](https://seqgen.com/contact/) - [BLOG](https://blog.seqgen.com) - [HOME](http://seqgen.com/) - [ABOUT US](https://seqgen.com/about-us/) - [Our History](https://seqgen.com/about-us/our-history-2/) - [Our Engineers](https://seqgen.com/about-us/our-engineers/) - INSTRUMENT MODEL - [LC/MS](https://seqgen.com/instrument-model/lc-ms/) - [Sciex 3000 system](https://seqgen.com/instrument-model/lc-ms/api-3000/) - [Sciex 3200 system](https://seqgen.com/instrument-model/lc-ms/api-3200/) - [Sciex 4000 system](https://seqgen.com/instrument-model/lc-ms/api-4000/) - [Sciex 4500 system](https://seqgen.com/instrument-model/lc-ms/api-4500/) - [Sciex 5500 system](https://seqgen.com/instrument-model/lc-ms/api-5500/) - [Sciex 6500 system](https://seqgen.com/instrument-model/lc-ms/api-6500/) - [DNA ANALYZER](https://seqgen.com/instrument-model/dna-analyzer/) - [ABI 3100](https://seqgen.com/instrument-model/dna-analyzer/abi-3100/) - [ABI 3130/3130XL](https://seqgen.com/instrument-model/dna-analyzer/abi-3130-3130xl/) - [ABI 3500/3500XL](https://seqgen.com/instrument-model/dna-analyzer/abi-3500-3500xl/) - [ABI 3730/3730XL](https://seqgen.com/instrument-model/dna-analyzer/abi-3730-3500xl/) - [REAL-TIME PCR](https://seqgen.com/instrument-model/real-time-pcr/) - [ABI 7300/7500](https://seqgen.com/instrument-model/real-time-pcr/abi-7300-7500/) - [ABI 7900](https://seqgen.com/instrument-model/real-time-pcr/abi-7900/) - [THERMAL CYCLERS](https://seqgen.com/instrument-model/thermal-cyclers/) - [ABI 2720](https://seqgen.com/instrument-model/thermal-cyclers/abi-2720-2/) - [ABI 9700](https://seqgen.com/instrument-model/thermal-cyclers/abi-9700/) - [ABI 9800](https://seqgen.com/instrument-model/thermal-cyclers/abi-9800/) - [LIQUID HANDLER](https://seqgen.com/instrument-model/liquid-handler/) - [Tecan Freedom Evo](https://seqgen.com/instrument-model/liquid-handler/tecan-freedom-evo/) - [SALES](https://seqgen.com/sales/) - [SERVICE](https://seqgen.com/service/) - [CONTACT](https://seqgen.com/contact/) - [SERVICE REQUEST](https://seqgen.com/service-request/) - [SALES INQUIRY](https://seqgen.com/sales-inquiry/) - [BLOG](https://blog.seqgen.com/) - [TESTIMONIALS](https://seqgen.com/testimonials/) - [ISO 9001 CERTIFICATE](https://seqgen.com/wp-content/uploads/2026/01/SeqGen-Final-Cert_2026-2029.pdf) ## [The Lab Savior: A Comprehensive HPLC Troubleshooting Guide](https://blog.seqgen.com/the-lab-savior-a-comprehensive-hplc-troubleshooting-guide) 09 March 2026 [![](https://blog.seqgen.com/hubfs/hplc-troubleshooting-sketchnote.png)](https://blog.seqgen.com/the-lab-savior-a-comprehensive-hplc-troubleshooting-guide) ## Quick-Start Diagnostic Summary  If you are currently facing an instrument issue, follow this logical flow to isolate the source: **Mobile Phase → Pump → Injector/Autosampler → Column → Detector**.   - **No Signal?** Verify the detector lamp is ON, check the wavelength setting, and purge the pump to remove air.  - **Pressure Anomalies?** High pressure usually indicates a blocked frit or column; low pressure often suggests a leak or air in the pump heads. - **Peak Shape Issues?** Address split peaks by replacing the guard column; resolve fronting by reducing injection volume; check fittings for dead volume if peaks are tailing.  - **Retention Time (RT) Shifts?** Stabilize the column oven temperature and ensure the mobile phase is fresh and degassed. - **Expert Maintenance:** [Contact SeqGen](https://seqgen.com/service-request/) for persistent Sciex MS sensitivity loss, internal Shimadzu failures, or annual Preventive Maintenance (PM)/compliance requirements.  [Read More](https://blog.seqgen.com/the-lab-savior-a-comprehensive-hplc-troubleshooting-guide) ## [Troubleshooting Vacuum Pressure Issues on the SCIEX Triple Quad 6500+](https://blog.seqgen.com/troubleshooting-vacuum-pressure-issues-on-the-sciex-triple-quad-6500-plus) 20 June 2025 [![](https://blog.seqgen.com/hubfs/sciex-6500plus-caution.png)](https://blog.seqgen.com/troubleshooting-vacuum-pressure-issues-on-the-sciex-triple-quad-6500-plus) Not reaching “Ready” on your [SCIEX Triple Quad 6500+](https://seqgen.com/instrument-model/lc-ms/api-6500/)? Vacuum pressure could be the culprit. Seeing flashing LEDs, high Torr readings, or sudden loss of sensitivity?You’re not alone—and the fix may be simpler than you think. This guide answers common questions about vacuum issues on the [SCIEX 6500+](https://seqgen.com/instrument-model/lc-ms/api-6500/) and walks you through quick checks to get your system back online. [Read More](https://blog.seqgen.com/troubleshooting-vacuum-pressure-issues-on-the-sciex-triple-quad-6500-plus) ## [Perform routine maintenance for mobile phases in the LCMS system](https://blog.seqgen.com/perform-routine-maintenance-for-mobile-phases-in-the-lcms-system) 11 April 2023 [![](https://blog.seqgen.com/hubfs/solvent-bottles-seqgen.png)](https://blog.seqgen.com/perform-routine-maintenance-for-mobile-phases-in-the-lcms-system) When it comes to liquid chromatography, the mobile phase plays a critical role in carrying the sample through the chromatography column and into the [mass spectrometer](https://sciex.com/products/mass-spectrometers). [Read More](https://blog.seqgen.com/perform-routine-maintenance-for-mobile-phases-in-the-lcms-system) ## [How to save Windows event logs in SCIEX Analyst software?](https://blog.seqgen.com/how-to-save-windows-event-logs-in-sciex-analyst-software) 09 March 2023 [![](https://blog.seqgen.com/hubfs/here-is-what-to-do-1.png)](https://blog.seqgen.com/how-to-save-windows-event-logs-in-sciex-analyst-software) [SCIEX Triple Quad](https://sciex.com/products/mass-spectrometers/triple-quad-systems) and [QTRAP systems](https://sciex.com/products/mass-spectrometers/qtrap-systems) are well known for their sensitivity and robustness in mass detection and quantification. But even with modern design, things could go wrong out of nowhere. [Read More](https://blog.seqgen.com/how-to-save-windows-event-logs-in-sciex-analyst-software) ## [Troubleshooting Mass Spectrometers 4500/5500/6500](https://blog.seqgen.com/troubleshooting-mass-spectrometers-4500/5500/6500) 17 February 2022 [![](https://blog.seqgen.com/hubfs/MS%20Services%20app.png)](https://blog.seqgen.com/troubleshooting-mass-spectrometers-4500/5500/6500) ## **Having issues activating your Mass Spectrometer profile?** Our expert team has helped many users to activate the profile in the hardware configuration. Troubleshooting your Mass Spectrometer is essential to figure out any issue when you cannot activate it.  [Read More](https://blog.seqgen.com/troubleshooting-mass-spectrometers-4500/5500/6500) [All posts](https://blog.seqgen.com/all) Search this site on Google Search Google ### Subscribe to Email Updates ## [![New Call-to-action](https://hubspot-no-cache-na2-prod.s3.amazonaws.com/cta/default/1939955/32eb2ce8-8e54-4e61-ad50-57f6b9b23d18.png)](https://hubspot-cta-redirect-na2-prod.s3.amazonaws.com/cta/redirect/1939955/32eb2ce8-8e54-4e61-ad50-57f6b9b23d18)  ### Recent Posts ### Posts by Topic - [DNA Analyzer (49)](https://blog.seqgen.com/topic/dna-analyzer) - [Others (42)](https://blog.seqgen.com/topic/others) - [Crossword Puzzle (11)](https://blog.seqgen.com/topic/crossword-puzzle) - [3730 Series Genetic Analyzer (10)](https://blog.seqgen.com/topic/3730-series-genetic-analyzer) - [Tecan Freedom Evo (10)](https://blog.seqgen.com/topic/tecan-freedom-evo) - [3500 Series Genetic Analyzer (6)](https://blog.seqgen.com/topic/3500-series-genetic-analyzer) - 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