Quick-Start Diagnostic Summary 

If you are currently facing an instrument issue, follow this logical flow to isolate the source: Mobile Phase → Pump → Injector/Autosampler → Column → Detector.  

• No Signal? Verify the detector lamp is ON, check the wavelength setting, and purge the pump to remove air. 
• Pressure Anomalies? High pressure usually indicates a blocked frit or column; low pressure often suggests a leak or air in the pump heads.
• Peak Shape Issues? Address split peaks by replacing the guard column; resolve fronting by reducing injection volume; check fittings for dead volume if peaks are tailing. 
• Retention Time (RT) Shifts? Stabilize the column oven temperature and ensure the mobile phase is fresh and degassed.
• Expert Maintenance: Contact SeqGen for persistent Sciex MS sensitivity loss, internal Shimadzu failures, or annual Preventive Maintenance (PM)/compliance requirements. 

Not reaching “Ready” on your SCIEX Triple Quad 6500+? Vacuum pressure could be the culprit. Seeing flashing LEDs, high Torr readings, or sudden loss of sensitivity? You’re not alone—and the fix may be simpler than you think. This guide answers common questions about vacuum issues on the SCIEX 6500+ and walks you through quick checks to get your system back online.

When it comes to liquid chromatography, the mobile phase plays a critical role in carrying the sample through the chromatography column and into the mass spectrometer.

SCIEX Triple Quad and QTRAP systems are well known for their sensitivity and robustness in mass detection and quantification. But even with modern design, things could go wrong out of nowhere.

Having issues activating your Mass Spectrometer profile?

Our expert team has helped many users to activate the profile in the hardware configuration. Troubleshooting your Mass Spectrometer is essential to figure out any issue when you cannot activate it.