--- title: SeqGen Blog | Real-Time PCR description: Real-Time PCR | --- - [MARKETPLACE](https://seqgen.com/marketplace/) - [CAREERS](https://seqgen.com/careers/) - [TERMS & CONDITIONS](https://seqgen.com/wp-content/uploads/2026/03/POL_011-Rev.A_Terms-and-Conditions-of-Sales-for-Products-and-Services.pdf) - [ISO 9001 CERTIFICATE](https://seqgen.com/wp-content/uploads/2026/01/SeqGen-Final-Cert_2026-2029.pdf) - [SALES INQUIRY](https://seqgen.com/sales-inquiry/) - [SERVICE REQUEST](http://seqgen.com/service-request/) - [TESTIMONIALS](https://seqgen.com/testimonials/) [![SeqGen](https://seqgen.com/wp-content/uploads/2020/03/seqgen_logo.png)](https://seqgen.com/) - [SeqMo Rewards](https://seqgen.com/seqmo-rewards/) - [HOME](https://seqgen.com/) - [ABOUT US](https://seqgen.com/about-us/) - [Our History](https://seqgen.com/about-us/our-history-2/) - [Our Engineers](https://seqgen.com/about-us/our-engineers/) - INSTRUMENT MODEL - [LC/MS](https://seqgen.com/instrument-model/lc-ms/) - [Sciex 3000 system](https://seqgen.com/instrument-model/lc-ms/api-3000/) - 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[ABI 7900](https://seqgen.com/instrument-model/real-time-pcr/abi-7900/) - [Thermal Cyclers](https://seqgen.com/instrument-model/thermal-cyclers/) - [ABI 2720](https://seqgen.com/instrument-model/thermal-cyclers/abi-2720-2/) - [ABI 9700](https://seqgen.com/instrument-model/thermal-cyclers/abi-9700/) - [ABI 9800](https://seqgen.com/instrument-model/thermal-cyclers/abi-9800/) - [Liquid Handler](https://seqgen.com/instrument-model/liquid-handler/) - [Tecan Freedom Evo](https://seqgen.com/instrument-model/liquid-handler/tecan-freedom-evo/) - [Microplate Reader](https://seqgen.com/instrument-model/microplate-reader/) - [Coming Soon](https://seqgen.com/instrument-models/microplate-reader/) - [SALES](https://seqgen.com/sales/) - [SERVICE](https://seqgen.com/service/) - [CONTACT](https://seqgen.com/contact/) - [BLOG](https://blog.seqgen.com) - [HOME](http://seqgen.com/) - [ABOUT US](https://seqgen.com/about-us/) - [Our History](https://seqgen.com/about-us/our-history-2/) - [Our Engineers](https://seqgen.com/about-us/our-engineers/) - INSTRUMENT MODEL - [LC/MS](https://seqgen.com/instrument-model/lc-ms/) - [Sciex 3000 system](https://seqgen.com/instrument-model/lc-ms/api-3000/) - [Sciex 3200 system](https://seqgen.com/instrument-model/lc-ms/api-3200/) - [Sciex 4000 system](https://seqgen.com/instrument-model/lc-ms/api-4000/) - [Sciex 4500 system](https://seqgen.com/instrument-model/lc-ms/api-4500/) - [Sciex 5500 system](https://seqgen.com/instrument-model/lc-ms/api-5500/) - [Sciex 6500 system](https://seqgen.com/instrument-model/lc-ms/api-6500/) - [DNA ANALYZER](https://seqgen.com/instrument-model/dna-analyzer/) - [ABI 3100](https://seqgen.com/instrument-model/dna-analyzer/abi-3100/) - [ABI 3130/3130XL](https://seqgen.com/instrument-model/dna-analyzer/abi-3130-3130xl/) - [ABI 3500/3500XL](https://seqgen.com/instrument-model/dna-analyzer/abi-3500-3500xl/) - [ABI 3730/3730XL](https://seqgen.com/instrument-model/dna-analyzer/abi-3730-3500xl/) - [REAL-TIME PCR](https://seqgen.com/instrument-model/real-time-pcr/) - [ABI 7300/7500](https://seqgen.com/instrument-model/real-time-pcr/abi-7300-7500/) - [ABI 7900](https://seqgen.com/instrument-model/real-time-pcr/abi-7900/) - [THERMAL CYCLERS](https://seqgen.com/instrument-model/thermal-cyclers/) - [ABI 2720](https://seqgen.com/instrument-model/thermal-cyclers/abi-2720-2/) - [ABI 9700](https://seqgen.com/instrument-model/thermal-cyclers/abi-9700/) - [ABI 9800](https://seqgen.com/instrument-model/thermal-cyclers/abi-9800/) - [LIQUID HANDLER](https://seqgen.com/instrument-model/liquid-handler/) - [Tecan Freedom Evo](https://seqgen.com/instrument-model/liquid-handler/tecan-freedom-evo/) - [SALES](https://seqgen.com/sales/) - [SERVICE](https://seqgen.com/service/) - [CONTACT](https://seqgen.com/contact/) - [SERVICE REQUEST](https://seqgen.com/service-request/) - [SALES INQUIRY](https://seqgen.com/sales-inquiry/) - [BLOG](https://blog.seqgen.com/) - [TESTIMONIALS](https://seqgen.com/testimonials/) - [ISO 9001 CERTIFICATE](https://seqgen.com/wp-content/uploads/2026/01/SeqGen-Final-Cert_2026-2029.pdf) ## [Did You Leave the Stove On?](https://blog.seqgen.com/did-you-leave-the-stove-on) 25 May 2017 [![](https://blog.seqgen.com/hubfs/IMG_20170523_135111.jpg)](https://blog.seqgen.com/did-you-leave-the-stove-on) [Read More](https://blog.seqgen.com/did-you-leave-the-stove-on) ## [qPCR “Base”ics](https://blog.seqgen.com/qpcr-baseics) 05 February 2016 ![qpcr1.png](https://blog.seqgen.com/hubfs/Blog_Images/qpcr1.png "qpcr1.png") Two weeks ago, during an unusually warm spell on the east coast, I received a text message from a friend who was suffering through the misfortune of a failed air conditioner. I was of course more than sympathetic as I sat in my nice cool home and insisted that she move her family into my guest bedroom. She politely declined and suffered through the calamity until she could get the unit replaced. Unfortunately she did not have a service contract and had to fork over a hefty chunk of her hard earned savings to replace the heat pump. [Read More](https://blog.seqgen.com/qpcr-baseics) ## [qPCR – The Rest of the Story…](https://blog.seqgen.com/qpcr-the-rest-of-the-story) 05 February 2016 ***If there is a 50-50 chance that something can go wrong, then 9 times out of ten it will.*– PAUL HARVEY, AMERican Journalist** [Read More](https://blog.seqgen.com/qpcr-the-rest-of-the-story) ## [To Compress or Not to Compress...](https://blog.seqgen.com/-to-compress-or-not-to-compress) 04 February 2016 ![seqgen\_compress.png](https://blog.seqgen.com/hs-fs/hubfs/Blog_Images/seqgen_compress.png?width=656&height=469&name=seqgen_compress.png "seqgen_compress.png") Have you ever had one of those annoying garden hoses that leaks no matter what you seem to do?  You change that little washer a zillion times but water is still erupting from one end or the other!  Similarly, this seems to be the topic of the month at SeqGen for our qPCR and thermal cycler customers.  Several calls and emails have come in detailing customer frustration over sample evaporation around the edges of their plates or lids cracking on the 9700 thermal cycler. [Read More](https://blog.seqgen.com/-to-compress-or-not-to-compress) [All posts](https://blog.seqgen.com/all) Search this site on Google Search Google ### Subscribe to Email Updates ## [![New Call-to-action](https://hubspot-no-cache-na2-prod.s3.amazonaws.com/cta/default/1939955/32eb2ce8-8e54-4e61-ad50-57f6b9b23d18.png)](https://hubspot-cta-redirect-na2-prod.s3.amazonaws.com/cta/redirect/1939955/32eb2ce8-8e54-4e61-ad50-57f6b9b23d18)  ### Recent Posts ### Posts by Topic - [DNA Analyzer (49)](https://blog.seqgen.com/topic/dna-analyzer) - [Others (42)](https://blog.seqgen.com/topic/others) - [Crossword Puzzle (11)](https://blog.seqgen.com/topic/crossword-puzzle) - [3730 Series Genetic Analyzer (10)](https://blog.seqgen.com/topic/3730-series-genetic-analyzer) - [Tecan Freedom Evo (10)](https://blog.seqgen.com/topic/tecan-freedom-evo) - [3500 Series Genetic Analyzer (6)](https://blog.seqgen.com/topic/3500-series-genetic-analyzer) - [AI 101 (6)](https://blog.seqgen.com/topic/ai-101) - [Archive (6)](https://blog.seqgen.com/topic/archive) - 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