As a result of the recent inquiries on assisting with COVID-19 tests, we are providing a few steps to help you identify what is needed. The FDA has approved many COVID-19 testing kits and some of them are compatible with the ABI 7500 regular, 7500 Fast, and 7500 Fast DX. Please consider the following instructions to assist you in choosing the correct one.
One of the most common complaints we hear from our customers is they are seeing less than optimal results in their sequencing data. After making sure your machine is working properly, we often come to the conclusion that the issue is related to the capillary array.
Most of the time, the problem is simply a dirty capillary array. Over time, dried and degraded polymer will accumulate on the inner walls of your capillary array. This can adversely affect the resolution during sample runs and cause your results to suffer. Some common symptoms of a failing array are:
- Loss of resolution
- Broadening or “fat” peaks
- Delayed DNA peaks
- Blue or yellow bands in some, but not all of the capillaries
- One or two capillaries showing no peaks at all