One of the most common complaints we hear from our customers is they are seeing less than optimal results in their sequencing data. After making sure your machine is working properly, we often come to the conclusion that the issue is related to the capillary array.

Most of the time, the problem is simply a dirty capillary array. Over time, dried and degraded polymer will accumulate on the inner walls of your capillary array. This can adversely affect the resolution during sample runs and cause your results to suffer. Some common symptoms of a failing array are:

• Loss of resolution
• Broadening or “fat” peaks
• Delayed DNA peaks
• Blue or yellow bands in some, but not all of the capillaries
• One or two capillaries showing no peaks at all

We strongly recommend to have a professional handle any instrument move to avoid compromising its performance and most importantly to avoid any accidents. Mishandling this machine can be very costly. Please note that our engineers are highly qualified and trained to perform decommission, recommission and to prepare your machine for a short and long distance move.

I'm taking a step back in time from our technology-driven culture. I have been, for the past two weeks, living like a pioneer. Ok, more like a part-time pioneer. My refrigerator decided to throw in the towel and call it quits three days before Easter. Before finally calling a technician for help, I tried the standard troubleshooting process, hoping it would provide resolution. 

I have a confession: I put up my Christmas tree in mid-November. Eager to fill my home with holiday cheer, I dragged my fake, pre-lit tree out of the attic, plugged it in, and expected pure bliss.  

SeqGen is incredibly dedicated to its customers and their machines. Your concerns are our concerns. We have recieved many emails and phone calls from our customers asking whether they have to upgrade their sequencing and fragment analysis software to work on the Windows 7 operating system. Like many major updates to these machines, this maintenance can be rather pricey. Before you write that check, you’ll want to make sure you actually need the update. 

Maybe it’s my obsessive behavior or maybe I’m paranoid, but as the seasons change, I feel the need to do thorough preparation on the home front. I pull out my trusty checklist of required maintenance and cleaning, then get to work.   

As the day wanes on, I find myself wondering, “What’s the point? Why do I exhaust myself fixing things that aren’t broken yet?” But then I remember Murphy’s Law – whatever can go wrong, will go wrong. If you simply anticipate and prepare for those inevitable little disasters, you can significantly reduce the amount of chaos you’ll have to deal with later. 

At SeqGen, we love sharing our best maintenance tips and tricks. The most rewarding part of running this blog is helping you learn more about your sequencer and how to care for it. Over the years, we've helped our end users troubleshoot all kinds of technical difficulties, from leaks to clogs to false error messages. But a lot of the questions we receive from customers circle back to the same problems. 

That's why we're diving into our archives this week and pulling our most popular, most requested blogs into one convenient post. After all, the classics never go out of style!

I am not a morning person. I don’t function well at o-dark-thirty. But thankfully, we have a wonderful invention called coffee. Each morning, I drag myself to the Keurig, place the cup under the spout and push start. One morning, I followed the same process and got nothing – barely any of the liquid cheer streamed out to fill my cup. Without proper care, the same thing could happen with your sequencer equipment. Instead of simply cleaning the outside of my Keurig and the cup holder, I needed to dig deeper and maintain the inside of the machine to keep my morning joy flowing steadily. 

It’s summertime, and for me, that means yard work. Or rather, my attempt at yard work. Last year was a scorcher, complete with water rations all over the country. But this year, I made a commitment to my yard to not let it die too much. To revive my brown paradise, I gave the task of watering to my five-year-old, who loves to soak everything and everyone in the process.

But when I went to turn off the hose, I was greeted by a massive pool of water, mud, mulch, and grass clippings just under the faucet. Clearly, water had been pushed in and out of the hose. However, I could tell a large amount had also leaked from the outdoor water faucet and started to build up. This gotme thinking — the same thing could happen with sequencer equipment.  

We have all seen those ridiculous internet images of chocolate chip cookies cooking on the dashboard of a car. But how ridiculous is that really? We all know that feeling when we climb into our hot summer car – seats scorching our legs, steering wheel unbearable to the touch, hot air blowing from the A/C unit. Now imagine how constant exposure to high temperatures and humidity could affect your laser performance.