How do you fix short read lengths on a 3730 analyzer? If read lengths drop (e.g., from 1000 bp to 750 bp), it is likely a polymer issue. To resolve this, perform a 60 °C warm water wash and install a fresh bottle of polymer. If this does not restore data quality, you likely have a faulty polymer from a defective production lot. Order a different lot number immediately, perform a second wash, and install the new lot.
If your Applied Biosystems (ABI) 3730 DNA Analyzer is stuck in initialization with that persistent yellow indicator light, don't panic. The fix might be as simple as replacing a cheap, little component: a fuse!
We strongly recommend to have a professional handle any instrument move to avoid compromising its performance and most importantly to avoid any accidents. Mishandling this machine can be very costly. Please note that our engineers are highly qualified and trained to perform decommission, recommission and to prepare your machine for a short and long distance move.
When your sequencer just stops in the middle of a run it can be flat-out scary. Everything was going fine, and now it seems like someone just hit your machine with a bat. We know that you probably immediately start thinking of the parts that you’ll need to replace, as well as the lost revenue and time. Before you reach for the checkbook and a bottle of migraine pills, hold on just a second. Let’s back up a few steps and attempt to get to the root cause of the issue.
When your autosampler stops prior to the completion of a run, check the event log to see what is going on. There are many possibilities for autosampler failure, and depending upon when the failure occurs and what the actual error message is, you may be able to salvage your data.
What is going on in the cosmos? We seem to be receiving a lot of calls and emails regarding the ABI 3730 autosampler. A very common error reported is: “Autosampler Detect a Non-assigned tray”.
This can be an especially tricky issue to deal with since the autosampler relies on many moving parts. To walk you through this problem and get your machine back up and running, we’ve compiled a few tips we’ve learned from dealing with these machines in the field.
The dog days of summer are here, and I don’t know about you but at times they can have me feeling burnt out! And just like us, our CE machines can burn out from time to time, too. If you’re seeing that annoying yellow light during initialization, it may be time to check your fuses on your ABI 3730 to see if they need to be replaced.
When we last left our troubled ABI 3730 user a week ago, she was suffering from blue background haze and had tried SeqGen’s previously mentioned tips to no avail. At this point we look for other reasons for the blue haze in her results.
As you may remember, the buffer, or in most cases the buffer make-up water, can cause a blue haze in your electropherograms. If you're having this problem, you need to check and see if there is any leak in the system.This is to make sure the buffer is not getting pulled into the pump piston and accidently passing into the capillary.
Sometimes running into problems can make us better people and build our character. Other times running into problems can make us want to pull our hair out. If you happen to run into a blue background on your array view, don’t stress just yet. Hopefully, this post will keep you from reaching for some Rogaine.
We all need a tune-up every once in a while, and this goes for PDP water trap in your 3730 as well. These PDP water traps should be flushed about once a month using distilled or deionized water.
By performing this procedure at least once a month, you’ll clear out any diluted polymer and bubbles. It’s best to leave the trap filled with the water. Doing this as a part of your regular maintenance will greatly decrease the chances of having a problem with your pump.
I do not know why this happens, but service issues seem to run in cycles at SeqGen. Lately we’ve been hearing quite a bit about your 3730 autosamplers. Maybe your machines are still “falling back”! At any rate, we would like to share some interesting tricks that may help you get those “pesky” autosamplers working again.
Let’s dig into two of the more common issues and discuss what might be the possible problems.
