The Black Screen of Death

31 August 2016

Dealing with sequencer problems is hard enough when your machine notifies you of what’s wrong. But working with a machine that won’t give you any information can make you want to crawl under a rock. 

A common service issue we see on 31xx and 3730 series machines is failure of the CCD camera. You may recognize this scenario: You arrive early Monday morning ready for a productive day of sequencing, load your plates, and then disaster strikes!  All that appears in the array viewer is a black screen. Panic mode sets in. After a deep calming breath, you email SeqGen the screen captures for our expert engineers to review and troubleshoot. Within 2 hours, SeqGen’s expert team responds by email and then by telephone:  “We are very sorry to report that your CCD camera has passed away.”  RIP, CCD Camera. You gave us some great years!

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How Will I Continue my ABI 3130 and 7900 Maintenance?

24 August 2016

If you are wondering about ongoing maintenance for your ABI 3130/xl Genetic Analyzer, or 7900HT qPCR, you aren’t alone. Thermofisher has announced that they will discontinue service for these machines in the not too distant future.

As a matter of fact, if you have one of these machines you’ve probably already gotten a notice saying that you’ll have to upgrade your technology if you’d like to continue maintenance. Lucky for you, SeqGen plans on covering these machines for many years to come!

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Is Your ABI 3730 Having Trouble Initializing? Time to Check The Fuses

17 August 2016

The dog days of summer are here, and I don’t know about you but at times they can have me feeling burnt out! And just like us, our CE machines can burn out from time to time, too. If you’re seeing that annoying yellow light during initialization, it may be time to check your fuses on your ABI 3730 to see if they need to be replaced. 

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Are your 3730 Results a Little Hazy? You May Have a Leak

10 August 2016

When we last left our troubled ABI 3730 user a week ago, she was suffering from blue background haze and had tried SeqGen’s previously mentioned tips to no avail. At this point we look for other reasons for the blue haze in her results.

As you may remember, the buffer, or in most cases the buffer make-up water, can cause a blue haze in your electropherograms. If you're having this problem, you need to check and see if there is any leak in the system.This is to make sure the buffer is not getting pulled into the pump piston and accidently passing into the capillary.



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Why Am I Seeing a Blue Background on My ABI 3730?

03 August 2016

Sometimes running into problems can make us better people and build our character. Other times running into problems can make us want to pull our hair out. If you happen to run into a blue background on your array view, don’t stress just yet. Hopefully, this post will keep you from reaching for some Rogaine. 

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