The Big, Ugly Green Monster Attacked Again. (Sequencer Producing a Green/Blue Background During Runs? Here’s How to Troubleshoot It)

27 April 2017

A SeqGen customer recently contacted us saying that they had noticed a green/blue background during sample runs and water plate runs. They had done a good job of trying to troubleshoot the issue themselves. After many failed attempts and lost time on their project, they threw in the towel and contacted SeqGen for a solution. We thought these troubleshooting efforts would be helpful if ever you experience the same issue.

Read More

What Should I Do If My MiSeq Fails to Measure Flow Rate When Starting Runs?

30 March 2017

We all love the MiSeq for the fast turnaround, remarkable data quality and its streamlined workflow. However, from time to time, our trusty machines will have an off day that can throw an elephant-sized wrench into your plans. From time to time, you may notice that you are unable to start your Miseq run because the pre-run flow check is failing.

Read More

Continuing Your 7900HT and 3130/xl Maintenance

22 March 2017

 

As new technology is developed and introduced to the marketplace, you may have heard a nasty rumor that the manufacturer will no longer be offering support or maintenance on older machines like the ABI 3130/xl Genetic Analyzer, or 7900HT qPCR.  You might have even gotten a notice that effectively says that you’ll have to upgrade to a newer model if you want to keep your lab operational.  Sure, these machines have improved functionality and can make a lab more efficient, but the bottom line is this unnecessary upgrade is costly and just doesn’t make sense for a lot of labs.

Read More

Your Sequencer Deserves a Spring Break Too

16 March 2017
It’s the start of that time of the year—vacation time!!! With spring almost upon us, it’s hard to concentrate when we are already envisioning ourselves basking in the warm sunshine. However, before you shut off the lights in the lab to enjoy some relaxing time away, it’s also important to shut down your machine properly so it’s ready to run upon your return.
Read More

Resuspending Your Samples In Water? Follow These 3 Precautions

08 March 2017

Many of our users have found that when using water for injections instead of Hi-Di formamide, they end up with issues that prevent base calling, even when following the machine standards. Proper technique should produce signal strengths well above the minimum threshold when samples are resuspended in formamide.

However, if you are still having issues with signal strength, you can try resuspending your samples in water, since this can increase signal strength by over 10x. If you do try employing water for injections, make sure you’re cautious of these three things:

Read More

4 Ways to Extend Your Lab's Budget Instantly

02 March 2017

No matter what industry you’re in, budgets are tight. Costs for reagents, coverage plans, unexpected breakdowns, and plenty of other issues--the funds dry up quickly.

However, over the years we’ve picked up plenty of tips and tricks that can save your lab money and extend the life of your machine and its parts. So without further ado, here are 4 ways you can squeeze more out of your lab’s budget now.

Read More

SeqGen is Now ISO 9001:2015 Certified

21 February 2017

There’s been a lot going on here at SeqGen recently. In addition to introducing our Next-Gen Sequencing Division, we’ve also been working on some internal processes to increase the efficiency of our service. And last month, all of our hard work was recognized when we received our International Organization for Standardization (ISO) 9001:2015 certification.

Read More

Re-Purposing Your Old Polymer

15 February 2017

We all know that the reagents for your sequencers and qPCR machines aren’t cheap, so of course everyone is looking for any and every way to save on these supplies. With a bottle of polymer costing a few hundred dollars, you learn quickly that it is not to be wasted. Luckily, some very bright people work with these machines every day and they’ve come up with some ingenious ways to get the most out of your polymer.

Read More

Is It Time for Your 3500 Genetic Analyzer's PM?

08 February 2017

With everything that goes on in the lab throughout the day, it’s easy to forget about routine maintenance and taking care of your machine. As with any piece of specialized equipment, you’re going to have wear and tear that causes a need for regular service. Without it, you’re risking unnecessary damage to your equipment and inaccurate sample runs.

If you have a coverage plan from SeqGen, you’ll receive one preventative maintenance visit per year, and just in case you forget about it, we’ll send you a reminder. We recommend that you let the professionals give your machine some tender love and care at least once a year, but in between service visits there are a few things you can do to prolong the life of your machine.

Read More

Optimizing MiSeq Library Cluster Density

02 February 2017

If you’ve been using your MiSeq for any length of time, you know that cluster density is a crucial part of NGS to generate the best and most data possible. Under-clustering and over-clustering your data will result in similar errors since your machine will not be able to find the best cluster focus in either case. In order to get the most data possible out of each of your runs, you’ll need to find your “sweet spot” to get the most usable data.

Read More

Subscribe to Email Updates

New Call-to-action 

Recent Posts

SeqGen is an independently owned and operated provider of repair services and refurbished laboratory equipment. Any product names, logos, brands, and other trademarks or images featured or referred to within the SeqGen website and/or on any social media forum are the property of their respective trademark holders or OEMs. These trademark holders are not affiliated with SeqGen or its website. These trademark holders do not sponsor or endorse SeqGen or any of its products or comments. The use of any trade name or trademark is for identification and reference purposes only and does not imply any association with the trademark holder of their product brand.