Making A Move? Don't Worry. Be Happy.

18 May 2017

 

Seriously, does anyone like to move? There isn’t enough pizza in the world to persuade your “friends” to borrow their pickup truck.  However, SeqGen is hired frequently to move lab equipment, and we love pizza.  We understand the importance of moving quickly and carefully in order to get your business back up and running smoothly as usual. Every move is different, but with each move we have become better equipped for the next.
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3730 Connection Check

11 May 2017

Your sequencer is stuck at yellow-light status. This means that the machine has not established a connection with the computer.

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The Forgotten Truth

04 May 2017

The famous line “If I only knew then what I know now” can mean a measure of savings down the road when it comes to your instruments.  In our busy lives there is a simple but forgotten cost-saving step, if you’re willing to do some regular maintenance. It may even end up saving you thousands in laser replacement costs or machine down time.

Your machine is manufactured with many interdependent parts that allow it to function properly. The laser is one of the sequencer’s most critical parts enabling you to complete runs, and the air filter could be the key to extending the life of the laser. 

The ABI 3130 and 3730 models have an air filter that helps remove contaminants before they reach the laser.  Between the routine laser maintenance and realignments performed, you can do one simple thing to help your machine: clean the air filter regularly. All you need is a $3 can of air from your local hardware store and the ability to follow these three simple steps.

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The Big, Ugly Green Monster Attacked Again. (Sequencer Producing a Green/Blue Background During Runs? Here’s How to Troubleshoot It)

27 April 2017

A SeqGen customer recently contacted us saying that they had noticed a green/blue background during sample runs and water plate runs. They had done a good job of trying to troubleshoot the issue themselves. After many failed attempts and lost time on their project, they threw in the towel and contacted SeqGen for a solution. We thought these troubleshooting efforts would be helpful if ever you experience the same issue.

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What Should I Do If My MiSeq Fails to Measure Flow Rate When Starting Runs?

30 March 2017

We all love the MiSeq for the fast turnaround, remarkable data quality and its streamlined workflow. However, from time to time, our trusty machines will have an off day that can throw an elephant-sized wrench into your plans. From time to time, you may notice that you are unable to start your Miseq run because the pre-run flow check is failing.

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Your Sequencer Deserves a Spring Break Too

16 March 2017
It’s the start of that time of the year—vacation time!!! With spring almost upon us, it’s hard to concentrate when we are already envisioning ourselves basking in the warm sunshine. However, before you shut off the lights in the lab to enjoy some relaxing time away, it’s also important to shut down your machine properly so it’s ready to run upon your return.
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Resuspending Your Samples In Water? Follow These 3 Precautions

08 March 2017

Many of our users have found that when using water for injections instead of Hi-Di formamide, they end up with issues that prevent base calling, even when following the machine standards. Proper technique should produce signal strengths well above the minimum threshold when samples are resuspended in formamide.

However, if you are still having issues with signal strength, you can try resuspending your samples in water, since this can increase signal strength by over 10x. If you do try employing water for injections, make sure you’re cautious of these three things:

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Our 5 Most Helpful Hints

12 January 2017

Over the past few years we’ve been bringing you the most common issues and best trouble shooting tips through our blog. There have been all kinds of odd errors for us to work on this year, but we kept seeing these particular problems occur over and over.

Since we love working with our partners in the lab, we wanted to dive into our blogs and bring you the most helpful tips and tricks we could find. So without further ado, here are the 5 most helpful hints we've given out so far.

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Z Axis Error? What is Your Autosampler Saying?

17 November 2016

When your sequencer just stops in the middle of a run it can be flat-out scary. Everything was going fine, and now it seems like someone just hit your machine with a bat. We know that you probably immediately start thinking of the parts that you’ll need to replace, as well as the lost revenue and time. Before you reach for the checkbook and a bottle of migraine pills, hold on just a second. Let’s back up a few steps and attempt to get to the root cause of the issue.

When your autosampler stops prior to the completion of a run, check the event log to see what is going on. There are many possibilities for autosampler failure, and depending upon when the failure occurs and what the actual error message is, you may be able to salvage your data.

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Still Running Windows XP?

10 November 2016

SeqGen is dedicated to our customers and your machines. Your concerns become our concerns. We have been receiving many emails and phone calls from our customers asking if they have to upgrade their sequencing and fragment analysis software to work on the Windows 7 operating system. Like many major updates with these machines, this can run you a pretty penny, and you’ll want to make sure you actually need the update before you blow your budget.

You may have gotten a notice from your manufacturer saying that in order to have the best security and efficiency for your machines, your software should be replaced to run under Windows 7. It may not sound like much of a setback; that is until they mention the multi thousands of dollars that you will need to find within your budget to upgrade your data collection software! THAT is quite the setback.

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